4NKN

The Crystal Structure of the N-terminal domain of COMMD9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	I 1 2 1
Unit cell lengths	89.690, 79.757, 131.164
Unit cell angles	90.00, 90.61, 90.00

Refinement procedure

Resolution	32.789 - 2.790
R-factor	0.2117
Rwork	0.210
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.396
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.460	39.460	2.940
High resolution limit [Å]	2.790	8.810	2.790
Rmerge	0.093	0.046	0.646
Total number of observations		5392	23049
Number of reflections	23054
<I/σ(I)>	16	42.8	3.2
Completeness [%]	98.6	99	91.6
Redundancy	3.8	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	20% PEG 3350, 0.2M Mg Nitrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K