4NIH
Crystal structure of AlkB E136L mutant protein with cofactors bound to dsDNA containing m6A/A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-01-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.072, 76.369, 53.178 |
Unit cell angles | 90.00, 108.98, 90.00 |
Refinement procedure
Resolution | 27.240 - 1.374 |
R-factor | 0.15987 |
Rwork | 0.159 |
R-free | 0.18003 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bie |
RMSD bond length | 0.007 |
RMSD bond angle | 1.337 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.7.0029) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.374 | 1.374 |
Number of reflections | 59661 | |
Completeness [%] | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG4000, 0.1 M sodium chloride, 0.05 M magnesium chloride, 0.1 M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |