4NI7
Crystal structure of human interleukin 6 in complex with a modified nucleotide aptamer (SOMAMER SL1025)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 32 |
| Unit cell lengths | 50.230, 50.230, 103.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.500 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26000 |
| Structure solution method | SAD WITH MR |
| Starting model (for MR) | 1il6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.922 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.460 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.614 |
| Number of reflections | 11411 | |
| <I/σ(I)> | 26.89 | 3.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 31% PEG 3350, 180 mM LiNO3, 100 mM Sodium Acetate (pH 5.5), 2.5% Hexamine Cobalt Chloride, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
