4NHR

Crystal structure of the outer membrane lipopolysaccharide transport protein LptE (RlpB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97890
Spacegroup name	P 65 2 2
Unit cell lengths	87.670, 87.670, 104.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.003 - 2.340
R-factor	0.2272
Rwork	0.226
R-free	0.24910
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.126
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.395	61.400
High resolution limit [Å]	2.170	2.340
Number of reflections	10493
<I/σ(I)>	19.3
Completeness [%]	100.0	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	100 mM Tris/HOAc pH 8.5, 200 mM KBr, 10% PEG 1000, 10% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 277K
2