4NH5

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 14-mer siRNA having 5'-pUU and UU-3' ends (2.55 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	118.286, 84.429, 51.049
Unit cell angles	90.00, 108.98, 90.00

Refinement procedure

Resolution	27.964 - 2.550
R-factor	0.2177
Rwork	0.211
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ngd
RMSD bond length	0.006
RMSD bond angle	0.978
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.640
High resolution limit [Å]	2.550	5.480	2.550
Rmerge	0.071	0.049	0.506
Number of reflections	15564
<I/σ(I)>	12.2
Completeness [%]	94.9	84.5	99.9
Redundancy	3	2.7	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M sodium formate, 0.1 M bicine, pH 8.1-8.5, 16-19% PEG5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K