4NGG

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 13-mer siRNA having 5'-A and UU-3' ends (2.6 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	I 2 2 2
Unit cell lengths	85.897, 97.240, 106.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.421 - 2.600
R-factor	0.1888
Rwork	0.186
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ngd
RMSD bond length	0.008
RMSD bond angle	1.094
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.088	0.036	0.722
Number of reflections	14296
<I/σ(I)>	9.5
Completeness [%]	99.9	99.3	100
Redundancy	9.7	8.9	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	0.2 M sodium chloride, 0.1 M sodium/potassium phosphate, pH 6.0-6.4, 10% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K