4NGC

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having UA-3' ends (2.1 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	109.081, 84.482, 51.550
Unit cell angles	90.00, 113.49, 90.00

Refinement procedure

Resolution	34.179 - 2.104
R-factor	0.1735
Rwork	0.172
R-free	0.20240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ngd
RMSD bond length	0.008
RMSD bond angle	1.121
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.065	0.043	0.214
Number of reflections	23726
<I/σ(I)>	9.4
Completeness [%]	95.2	99.1	75.8
Redundancy	4.9	5.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M sodium/potassium tartrate, 0.1 M Bis-Tris propane, pH 7.0, 18-22% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K