4NG8
Dialyzed HEW lysozyme batch crystallized in 1.9 M CsCl and collected at 100 K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.935, 78.935, 37.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.820 - 1.090 |
| R-factor | 0.155 |
| Rwork | 0.152 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 1.160 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Number of reflections | 91904 | |
| <I/σ(I)> | 15.55 | 3.47 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Dialyzed lysozyme, no buffer added, 1.9 M CsCl, pH 4.5, Batch crystallization, temperature 293K |
