4NG2

Crystal structure of LasR LBD-QslA complex from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	163.507, 185.887, 56.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.033 - 2.413
R-factor	0.2337
Rwork	0.231
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2uv0
RMSD bond length	0.010
RMSD bond angle	1.325
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.033	2.540
High resolution limit [Å]	2.410	2.410
Number of reflections	64498
Completeness [%]	96.5	78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	PEG 1K, 0.2M MgCl2, 0.1M NaCl, 50mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K