4NFU

Structure of the central plant immunity signaling node EDS1 in complex with its interaction partner SAG101

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0399, 1.0404, 1.0313, 0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	112.586, 113.643, 125.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.278 - 2.210
R-factor	0.1768
Rwork	0.176
R-free	0.19550
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.623
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP (INTEGRATED INTO AUTO-RICKSHAW (EMBL-Hamburg))
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.250
High resolution limit [Å]	2.210	2.210
Rmerge	0.054	0.972
Number of reflections	78935
<I/σ(I)>	21.6	2
Completeness [%]	97.3	79.1
Redundancy	7.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, pH 7.5, 5% w/v PEG4000, 5% 2-propanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K