4NE3

Human MHF1-MHF2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	61.969, 52.945, 60.283
Unit cell angles	90.00, 114.91, 90.00

Refinement procedure

Resolution	28.102 - 1.801
R-factor	0.2324
Rwork	0.231
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.928
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.102
High resolution limit [Å]	1.801
Number of reflections	16358
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1