4NDK

Crystal structure of a computational designed engrailed homeodomain variant fused with YFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	150
Detector technology	PIXEL
Collection date	2009-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033200
Spacegroup name	C 2 2 21
Unit cell lengths	75.890, 192.650, 107.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 2.300
R-factor	0.1929
Rwork	0.190
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.155
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	34.000
High resolution limit [Å]	2.300
Number of reflections	35208

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1 M sodium chloride, 12% v/v 2-propanol, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K