4NBT

Crystal structure of FabG from Acholeplasma laidlawii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Detector technology	CCD
Collection date	2012-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	61.968, 62.364, 66.765
Unit cell angles	66.03, 76.63, 79.93

Refinement procedure

Resolution	39.487 - 1.480
R-factor	0.1285
Rwork	0.128
R-free	0.15400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q7b
RMSD bond length	0.009
RMSD bond angle	1.385
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.487
High resolution limit [Å]	1.480	1.480
Number of reflections	142273
Completeness [%]	96.4	96.43

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M Ammonium fluoride and 20% PEG 3,350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K