4NAW
Crystal Structure of Human ATG12~ATG5-ATG16N in complex with a fragment of ATG3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.584, 120.097, 189.466 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.832 - 2.195 |
R-factor | 0.1723 |
Rwork | 0.172 |
R-free | 0.21530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.760 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.195 | 4.740 | 2.195 |
Rmerge | 0.091 | 0.037 | 0.859 |
Number of reflections | 109340 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 99.9 | 100 | 99.4 |
Redundancy | 7 | 7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PROTEIN - 10 MG/ML in 0.01M HEPES pH 7.0, 0.15 M NaCl, 0.001 M DTT, RESERVOIR - 0.1M MES pH6.0-6.75, 0.2 M ammonium sulfate, 10-16% PEG 5000 MME, vapor diffusion, sitting drop, temperature 293K |