4NAE
PcrB from Geobacillus kaustophilus, with bound G1P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Detector technology | CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.350, 157.970, 38.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.416 - 2.000 |
| R-factor | 0.2043 |
| Rwork | 0.202 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.157 |
| Refinement software | PHENIX ((phenix.refine: dev_1422)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.038 |
| Number of reflections | 151551 |
| Completeness [%] | 80.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 19% w/v PEG4000, VAPOR DIFFUSION, HANGING DROP |
