4NAD
Crystal Structure of the C-terminal Domain of CREPT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.280, 55.660, 84.450 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 42.171 - 2.800 |
| R-factor | 0.2396 |
| Rwork | 0.237 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.565 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.171 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 14346 | |
| Completeness [%] | 96.4 | 96.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 28% PEG 3350, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
