4NAA
Crystal structure of UVB photoreceptor UVR8 from Arabidopsis thaliana and UV-induced structural changes at 120K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-12 |
Detector | RAYONIX MX-300 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.521, 77.336, 189.312 |
Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
Resolution | 31.090 - 1.670 |
R-factor | 0.149 |
Rwork | 0.147 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dnw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.046 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.670 |
Rmerge | 0.039 |
Number of reflections | 154616 |
<I/σ(I)> | 23 |
Completeness [%] | 82.8 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9.2 | 290 | protein 8mg/ml, 0.1M MgCl2 15-18% PEG8000 and 0.1M Tris HCl, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |