4N9I
Crystal Structure of Transcription regulation protein CRP complexed with cGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.568, 56.171, 186.718 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 42.570 - 2.190 |
| R-factor | 0.17154 |
| Rwork | 0.170 |
| R-free | 0.20784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hif |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Rmerge | 0.066 | 0.147 |
| Number of reflections | 45063 | |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG 400, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
