4N8M
Structural polymorphism in the N-terminal oligomerization domain of NPM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.675, 69.794, 88.630 |
| Unit cell angles | 90.00, 99.87, 90.00 |
Refinement procedure
| Resolution | 37.260 - 1.802 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 49059 | |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 97.4 | 83.7 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | 0.6M 1,6 hexandiol, 10mM cobalt chloride, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, temperature 291K |
