4N8K
E249A mutant, RipA structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.182, 108.176, 84.987 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 43.585 - 2.000 |
| R-factor | 0.1487 |
| Rwork | 0.148 |
| R-free | 0.17590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qlk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.071 | 0.033 | 0.442 |
| Number of reflections | 62152 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.3 | 99.9 | 97 |
| Redundancy | 3.8 | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M BisTris pH 5.5, 21% PEG 3350, 0.2M Ammonium sulfate, 1 mM succinyl CoA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
