4N7Z

Crystal structure of human Plk4 cryptic polo box (CPB) in complex with a Cep192 N-terminal fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	67.308, 67.308, 287.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.145 - 2.850
R-factor	0.2324
Rwork	0.234
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g7n
RMSD bond length	0.015
RMSD bond angle	1.467
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	2.900	2.800
High resolution limit [Å]	2.750	2.850	2.750
Rmerge	0.115	0.574	0.838
Number of reflections	10857
<I/σ(I)>	15.9	3.13	1.65
Completeness [%]	98.8	99	94.3
Redundancy	9.5	8.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295.15	20% PEG 1500, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K