4N7I
Crystal Structure of Intracellular B30.2 Domain of BTN3A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-20 |
| Detector | MAR scanner 225mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.032, 44.957, 124.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.030 - 1.401 |
| R-factor | 0.1367 |
| Rwork | 0.135 |
| R-free | 0.16640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wl1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.969 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
| Number of reflections | 42349 | ||
| Completeness [%] | 96.2 | 93.3 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapour diffusion, Sitting drop | 7.2 | 298 | 0.2M MgCl2, 22%PEG3350, pH 7.2, vapour diffusion, Sitting drop, temperature 298K |
