4N7H

Crystal Structure of the Complex of 3rd WW domain of Human Nedd4 and 1st PPXY Motif of ARRDC3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-21
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	38.882, 38.882, 49.997
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.673 - 1.698
R-factor	0.1907
Rwork	0.189
R-free	0.23310
RMSD bond length	0.011
RMSD bond angle	1.291
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.068	0.055	0.210
Number of reflections	5129
<I/σ(I)>	24.5
Completeness [%]	99.6	97.9	99.4
Redundancy	13.3	11.8	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.35M (NH4)SO4, 100mM Tris-HCl 8.0, 100mM Guanidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 294K