4N7G
Crystal structure of 14-3-3zeta in complex with a peptide derived from ExoS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 105.420, 105.420, 114.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.440 - 2.250 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.22290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.026 |
RMSD bond angle | 2.644 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.440 | 2.300 | |
High resolution limit [Å] | 2.250 | 12.000 | 2.250 |
Rmerge | 0.114 | 0.013 | 0.013 |
Number of reflections | 35293 | 273 | 2203 |
<I/σ(I)> | 19.69 | 39.97 | 4.46 |
Completeness [%] | 100.0 | 96.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277.15 | 0.2 M sodium chloride, 0.1 M sodium acetate, pH 4.5, 40% v/v PEG300, VAPOR DIFFUSION, temperature 277.15K |