4N77
Crystal structure of Cas protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.618, 62.618, 93.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.003 - 1.999 |
| R-factor | 0.1723 |
| Rwork | 0.169 |
| R-free | 0.23120 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.216 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.999 | 1.999 |
| Rmerge | 0.120 | 0.511 |
| Number of reflections | 13127 | |
| <I/σ(I)> | 23.5 | 5 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris.HCl, 5% PEG8000, 28% PEG300, 10% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris.HCl, 5% PEG8000, 28% PEG300, 10% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
