4N6Q

Crystal structure of VosA velvet domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2010-10-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 41 2 2
Unit cell lengths	45.400, 45.400, 189.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.100 - 1.790
R-factor	0.1851
Rwork	0.183
R-free	0.22030
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.567
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.100	8.000	1.840
High resolution limit [Å]	1.790	5.660	1.790
Rmerge	0.036	0.024	0.286
Number of reflections	19471	721	2440
<I/σ(I)>	22.17	47.47	4.64
Completeness [%]	99.2	100	92
Redundancy	4.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100 mM MES, pH 6.5, 32% PEG 2000 MME, 150 mM KI, VAPOR DIFFUSION, SITTING DROP, temperature 293K