4N6M
Crystal structure of human cystatin E/M produced in LEXSY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.710, 82.630, 43.490 |
| Unit cell angles | 90.00, 112.81, 90.00 |
Refinement procedure
| Resolution | 40.120 - 2.900 |
| R-factor | 0.2607 |
| Rwork | 0.261 |
| R-free | 0.28346 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n6l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.814 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 2.450 |
| High resolution limit [Å] | 2.300 | 2.330 |
| Number of reflections | 10699 | |
| Completeness [%] | 95.5 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 22 % PEG 8000, 100 mM MES sodium salt pH 6.5, 200 mM ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
