4N6L
Crystal structure of human cystatin E/M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.730, 68.600, 40.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.269 - 1.952 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gax |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.941 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.600 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 8955 | |
| Completeness [%] | 98.9 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30 % PEG 4000, 100 mM sodium acetate pH 4.6, 200 mM ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
