4N5Z
Crystal structure of aerosol transmissible influenza H5 hemagglutinin mutant (N158D, N224K, Q226L and T318I) from the influenza virus A/Viet Nam/1203/2004 (H5N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.05876 |
Spacegroup name | P 1 |
Unit cell lengths | 117.240, 118.943, 273.410 |
Unit cell angles | 88.43, 89.68, 60.26 |
Refinement procedure
Resolution | 47.749 - 2.954 |
R-factor | 0.2354 |
Rwork | 0.233 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n5y |
RMSD bond length | 0.011 |
RMSD bond angle | 1.455 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.020 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 236232 | |
<I/σ(I)> | 8.5 | |
Completeness [%] | 89.7 | 77.4 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1 M tris, pH 8.0, 0.2 M KF, 20% (w/v) PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |