4N57
Crystal structure of aminoglycoside phosphotransferase APH(2'')-IVa ADP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.051, 65.802, 76.731 |
Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
Resolution | 49.931 - 2.350 |
R-factor | 0.1852 |
Rwork | 0.182 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n4v |
RMSD bond length | 0.009 |
RMSD bond angle | 1.189 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.980 | 49.980 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.067 | 0.067 |
Number of reflections | 30125 | |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 291 | 75 mM Ammonium citrate 12% PEG3350, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |