4N4Y
Structure of Recombinant Cytochrome ba3 Oxidase mutant G232V from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 144.150, 98.400, 114.100 |
Unit cell angles | 90.00, 138.93, 90.00 |
Refinement procedure
Resolution | 74.960 - 2.900 |
R-factor | 0.17905 |
Rwork | 0.177 |
R-free | 0.22531 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.518 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.960 | |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 21798 | |
Completeness [%] | 98.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 50 mM sodium cacodylate, pH 6.5, 1.6 M sodium chloride, 40% PEG400, LIPIDIC CUBIC PHASE, temperature 293K |