4N3G

Crystal structure of eukaryotic translation initiation factor eIF5B (870-1116) from Chaetomium thermophilum, domains III and IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826580
Spacegroup name	P 31 2 1
Unit cell lengths	98.230, 98.230, 97.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.857 - 3.203
R-factor	0.1915
Rwork	0.190
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.611
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.795		3.300
High resolution limit [Å]	3.200	50.000	3.200
Rmerge	0.034		0.574
Number of reflections	9288		800
<I/σ(I)>	32.12		4.31
Completeness [%]	99.8		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	100 mM MES, 12% PEG 20000, 10 mM Na-lactate, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K