4N3D
Crystal structure of the dimeric variant EGFP-K162Q in P61 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 121.861, 121.861, 64.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.210 - 1.340 |
| R-factor | 0.1807 |
| Rwork | 0.181 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.041 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.390 |
| High resolution limit [Å] | 1.340 | 2.890 | 1.340 |
| Rmerge | 0.053 | 0.026 | 0.659 |
| Number of reflections | 122157 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 99.3 | 99.7 | 93.3 |
| Redundancy | 7.4 | 7.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Two microliters of protein 10mg/ml in buffer 20 Tris 8.0 100 NaCl mixed with equal amount of resevoir solution - 0.056 NaH2PO4, 1.344 M K2HPO4 pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
