4N2O
Structure of a novel autonomous cohesin protein from Ruminococcus flavefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.240, 67.726, 258.907 |
| Unit cell angles | 90.00, 93.08, 90.00 |
Refinement procedure
| Resolution | 22.770 - 2.442 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.22889 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.770 |
| High resolution limit [Å] | 2.440 |
| Rmerge | 0.140 |
| Number of reflections | 72856 |
| <I/σ(I)> | 24 |
| Completeness [%] | 97.8 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 9 ul drop containing 2 ul of SeMet-RfCohG-CH solution (13mg/ml) and 7 ul reservoir solution (0.1 M Sodium Acetate pH 5 and 6% w/v Polyethylene glycol 4000), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
