4N25
Crystal structure of Protein Arginine Deiminase 2 (250 uM Ca2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Detector technology | CCD |
Collection date | 2004-01-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.276, 51.536, 75.992 |
Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
Resolution | 36.603 - 1.931 |
R-factor | 0.1811 |
Rwork | 0.179 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n20 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.221 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.603 | |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.084 | 0.436 |
Number of reflections | 54984 | |
<I/σ(I)> | 16.5 | 2 |
Completeness [%] | 96.6 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 10-20% MPD, 50 mM MES, pH 5.6, 0.12 M sodium acetate, VAPOR DIFFUSION, SITTING DROP |