4N21
Crystal structure of the GP2 Core Domain from the California Academy of Science Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.676, 46.713, 109.451 |
| Unit cell angles | 102.32, 89.99, 119.98 |
Refinement procedure
| Resolution | 39.220 - 1.990 |
| R-factor | 0.1704 |
| Rwork | 0.168 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g2k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.148 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.059 | 0.041 | 0.447 |
| Number of reflections | 51572 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 97.7 | 96.8 | 96.9 |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M lithium sulfate, 0.1 M MES-NaOH, pH 6, 35% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
