4N1K

Crystal structures of NLRP14 pyrin domain reveal a conformational switch mechanism, regulating its molecular interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-03
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 63
Unit cell lengths	89.210, 89.210, 106.571
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.000 - 3.000
R-factor	0.2162
Rwork	0.214
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NLRP14 WT Ser6-Pro67
RMSD bond length	0.002
RMSD bond angle	0.811
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.540
High resolution limit [Å]	3.000
Number of reflections	9722
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.2 M Cesium chloride and 2.2 M Ammonium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K