4N1H
Structure of a single-domain camelid antibody fragment cAb-F11N in complex with the BlaP beta-lactamase from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.088, 89.821, 75.749 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 75.440 - 3.000 |
| R-factor | 0.2122 |
| Rwork | 0.208 |
| R-free | 0.28449 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m3j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.440 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.185 | 0.505 |
| Number of reflections | 15849 | |
| <I/σ(I)> | 5.4 | 2.4 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 uL acetate 0.1 M, pH 4.6, 12 % P4K + 0.1 uL protein complex at 14 mg/mL in Tris 20 mM, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
