4N0J
Crystal structure of dimethyllysine hen egg-white lysozyme in complex with sclx4 at 1.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 198.8 |
| Detector technology | CCD |
| Collection date | 2012-12-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.850, 30.200, 94.210 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 31.200 - 1.900 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4IPQ |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.194 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.220 | 1.700 |
| High resolution limit [Å] | 1.674 | 1.670 |
| Rmerge | 0.116 | 1.026 |
| Number of reflections | 27755 | |
| <I/σ(I)> | 6.8 | 1.1 |
| Completeness [%] | 92.0 | 86.4 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 293.15 | 6 % PEG 8000, 100 mM, MAGNESIUM CHLORIDE, 50 mM SODIUM CHLORIDE, 100 mM SODIUM CACODYLATE, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
