4N08
Structure of Trypanosoma brucei brucei adenosine kinase (apo)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97630 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 60.084, 60.084, 192.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.900 - 2.600 |
R-factor | 0.16999 |
Rwork | 0.167 |
R-free | 0.23082 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.465 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 57.360 |
High resolution limit [Å] | 2.600 |
Number of reflections | 11649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 0.1 M Tris, pH 8.8, 60% (v/v) MPD, VAPOR DIFFUSION, HANGING DROP, temperature 277K |