4N04
The crystal structure of glyoxalase / bleomycin resistance protein from Catenulispora Acidiphila DSM 44928
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9789900 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 123.600, 123.600, 123.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.086 - 2.489 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.22740 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 11068 | |
| <I/σ(I)> | 21.5 | 6.6 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 18.7 | 19.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 0.1 M CHES:NaOH pH 9.5 1.0 M Sodium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
