4N01
The crystal structure of a periplasmic binding protein from Veillonella parvula dsm 2008
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9789666 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.192, 46.330, 108.550 |
Unit cell angles | 90.00, 119.62, 90.00 |
Refinement procedure
Resolution | 28.141 - 1.797 |
R-factor | 0.1728 |
Rwork | 0.171 |
R-free | 0.20450 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.982 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 82694 | |
<I/σ(I)> | 8.1 | 2.19 |
Completeness [%] | 99.6 | 99 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | 0.2 M Ammonium Formate pH 6.6, 20% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |