4MZH

Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2s) peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77
Detector technology	CCD
Collection date	2012-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 2
Unit cell lengths	122.985, 41.315, 50.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.008 - 2.204
R-factor	0.2014
Rwork	0.196
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.870	2.240
High resolution limit [Å]	2.200	2.770	2.200
Rmerge	0.072	0.144	0.612
Number of reflections	13269
<I/σ(I)>	21.46	14.11	2.98
Completeness [%]	97.8	98.5	97.5
Redundancy	4.4	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	20% PEG 8000, 20% PEG 400, 0.1M MgCl2, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K