4MYW
Structure of HSV-2 gD bound to nectin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 53.337, 170.857, 192.251 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.930 - 3.189 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u82 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.189 | 3.200 |
| Rmerge | 0.091 | 0.492 |
| Number of reflections | 30129 | |
| <I/σ(I)> | 15.672 | 2.884 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.2M sodium chloride, 0.1M sodium cacodylate, 8% w/v PEG 8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
