4MYG
MAPK13, active form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2010-08-24 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0007 |
| Spacegroup name | P 65 |
| Unit cell lengths | 107.232, 107.232, 125.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.307 - 2.594 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cm8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.594 | 2.594 |
| Rmerge | 0.088 | 0.590 |
| Number of reflections | 24975 | |
| <I/σ(I)> | 19.8 | 1.96 |
| Completeness [%] | 98.1 | 90.3 |
| Redundancy | 8.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 290 | 100 mM Bis-Tris, 21% PEG3350, 200 mM sodium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
