4MYC
Structure of the mitochondrial ABC transporter, Atm1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-08-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 156.618, 156.618, 520.562 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.600 - 3.060 |
R-factor | 0.253 |
Rwork | 0.251 |
R-free | 0.29790 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.354 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXCD |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.600 |
High resolution limit [Å] | 3.060 |
Number of reflections | 76568 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | 35% (w/v) PEE (pentaerythritol ethoxylate), 100mM MES, pH 6.5, 200mM ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 283K |