4MY5

Crystal structure of the aromatic amino acid aminotransferase from Streptococcus mutants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	104.754, 64.257, 125.600
Unit cell angles	90.00, 106.77, 90.00

Refinement procedure

Resolution	52.493 - 2.194
R-factor	0.1934
Rwork	0.191
R-free	0.23880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.690	56.690	2.270
High resolution limit [Å]	2.190	4.720	2.190
Rmerge	0.069
Number of reflections	76921
Completeness [%]	93.0	97	60.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.2M potassium chloride pH 7.0, 20%(w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K