4MV9
Crystal Structure of Biotin Carboxylase from Haemophilus influenzae in Complex with Bicarbonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 64 |
| Unit cell lengths | 85.628, 85.628, 102.738 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.876 - 1.980 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.952 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.230 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 29598 | |
| <I/σ(I)> | 16.2 | 2.3 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.2 M KF, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295.15K |
