4MU8
Crystal structure of an oxidized form of yeast iso-1-cytochrome c at pH 8.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 93.621, 93.621, 155.014 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.998 - 1.450 |
| R-factor | 0.1455 |
| Rwork | 0.145 |
| R-free | 0.15380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ycc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 71415 | |
| <I/σ(I)> | 14.16 | 2.43 |
| Completeness [%] | 99.9 | 99.23 |
| Redundancy | 9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 90% ammonia sulfate, 0.1 M Tris-HCl (pH 8.8), 4% tert-butanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
