4MU5

Crystal structure of murine neuroglobin mutant M144W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-12
Detector	PSI PILATUS 6M
Wavelength(s)	0.932
Spacegroup name	H 3 2
Unit cell lengths	87.636, 87.636, 115.217
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.887 - 1.800
R-factor	0.2011
Rwork	0.199
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.431
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.890	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	15838
Completeness [%]	98.9	98.05

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1M MES, pH6.5, 1.6M AS, 10% dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 294K